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GFPIII General Fluorescence Probe
The GFPIII is a general purpose modulated fiber optic probe fluorometer designed for a variety of measurement applications. It is available with a choice of excitation and detection modules to meet most fluorescence measurement requirements including popular selections such as; Chlorophyll, Rhodamine, Fluorescein, GFP, and most other Fluorescent protein compounds. Custom modules can also be created for special applications, with filters available from the UV to the near infrared range.
GFPIII is a general purpose fluorescence probe a New Standard in Fibre Optic Fluorometers
The GFPIII is a general purpose fluorescence probe designed for a wide variety of measurement applications. It’s flexible fibre optic probe enables highly sensitive detection for both in-situ and in-vitro environments. Detection is capable down to at least 10 ppb of fluorescein in pure water.
This compact hand-held instrument includes on-board data logging and is capable of single and continuous measuring modes. User changeable modules are available to detect most fluorescent markers and dyes including: GFP & other Fluorescent Proteins, Fluorescein, Rhodamine, Chlorophyll, Brighteners, and much more.
• Detection of GFP, green fluorescent protein
• Detection of most other fluorescent proteins
• Detection of specialised fluorescent compounds down to the ppb range
• Transgenic crop research & development
• Detect genetically modified organisms
• Gene expression studies
• Detection & measurement of contaminants
• Pesticide / Chemical spray analysis
• Parts inspection
As a hand-held, field-portable instrument, the lightweight and easy to carry GFPIII incorporates on-board data logging and a bright TFT color graphics touch sensitive display screen for system parameters and data display. Up to two gigabytes of data, traces, calibration tables, and user settings can be stored on the instrument. Rechargeable AA batteries will commonly allow the instrument to run for up to eight hours before recharging. A second set of batteries are included with the instrument. A metal leaf clip is provided for those doing transgenic and GFP detection
studies as well.
Measured Parameters: User programmable, can calibrate to known chemical concentrations or ratios. The number of calibration tables and measurement parameter descriptors are only limited by the memory capacity.
Resolution: User defined down to 1 ppb.
Sensitivity: Fluoroprobe dependent. For example; 10ppb for Fluorescein in pure water and 30ppb for Rhodamine in pure water.
Source: (1) LED (selected for application) LEDs range from 375nm to 660nm. User changeable.
Detector: Solid state, high sensitivity detector. Band limiting filter sets provided. Filters set the detection range. Wavelengths available from 400nm to 750nm. User changeable.
Detection: Modulated light digitally controlled to minimize background detection. Temperature compensation included for light source and detector.
User selectable single measuring and continuous measuring modes. The user selects a mode for single measurement mode, or a continuous measurement mode that allows sampling from one per second to one per minute. The continuous mode allows unattended measuring for up to four hours. The results are recorded in a measuring file and they are graphically displayed on the measuring screen.
Opti-Sciences has designed this instrument to provide the most reliable results available by including temperature compensation and multi-point calibration capabilities over the measuring range selected.
GFPIII General Fluorescence Probe